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The reactions were quenched by boiling with Laemmli sample buffer for 5 min

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Siddiqi AK, Khan MS, Kulkarni A, Hall ME, Bhm M, Dez J, et al

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Estrogens well-documented role in the brain has far-reaching implications for both emotional regulation and cognitive function, making its loss during menopause a critical factor in the emergence of these mental health disturbances [39]

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