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100 L of supernatant was transferred to a black opaque flat-bottom 96-well-plate (Nunc) and fluorescence was read on a Synergy 2 plate reader (BioTek) with 485 nm excitation/528 nm emission filter set
E.SchreibeltG.Vriesd
In addition to its effects on neurotransmitter systems and neuroplasticity, estrogen interacts with inflammation and oxidative stress pathways
It is a safe, convenient option with only minor potential side effects, such as slight discomfort at the injection site or mild lightheadedness
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