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Gilmore LA, Klempel-Donchenko M, Redman LM

To obtain a GLP-1RG s complex with good homogeneity and stability, we used the NanoBiT tethering strategy, in which the C-terminus of rat G1 was linked to HiBiT subunit with a 15-amino acid polypeptide (GSSGGGGSGGGGSSG) linker and the C-terminus of GLP-1R was directly attached to LgBiT subunit followed by a TEV protease cleavage site and a double MBP tag (Supplementary Fig

The microneedle manufacturing technology preserves the integrity of the biodegradable needles, which are sensitive to moisture and impact, until they are used